細(xì)胞名稱

人肝癌細(xì)胞HepG2                  

貨物編碼

BFN60800692

產(chǎn)品規(guī)格

T25培養(yǎng)瓶x1

1.5ml凍存管x2

細(xì)胞數(shù)量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運(yùn)輸方式

常溫保溫運(yùn)輸

干冰運(yùn)輸

安全等級(jí)

1

用途限制

僅供科研用途               1類

培養(yǎng)體系

DMEM高糖培養(yǎng)基（Hyclone）+10%胎牛血清（Gibco）+1%雙抗（Hyclone）

培養(yǎng)溫度

37℃

二氧化碳濃度

5%

簡(jiǎn)介

人肝癌細(xì)胞HepG2細(xì)胞來(lái)源于一名15歲的白人少年的肝癌組織。該細(xì)胞表達(dá)甲胎蛋白、白蛋白、α-2-巨球蛋白、α-1-抗胰蛋白酶、轉(zhuǎn)鐵蛋白、α-1-抗凝乳蛋白酶、結(jié)合珠蛋白、銅藍(lán)蛋白、纖溶酶原、補(bǔ)體C4、C3激活物、纖維蛋白原、α-1酸性糖蛋白、α-2-HS-糖蛋白、β-脂蛋白、視黃醇結(jié)合蛋白；表達(dá)胰島素受體和胰島素樣生長(zhǎng)因子IGFⅡ的受體；該細(xì)胞具有3-羥基-3-甲酰輔酶A還原酶和肝甘油三酯脂肪酶的活性。目前尚未證明該細(xì)胞中有HBV基因組。 人肝癌細(xì)胞HepG2細(xì)胞由青旗（上海）生物技術(shù)發(fā)展有限公司于2019年引種自ATCC（HB-8065)。

注釋

Problematic cell line: Misidentified. Originally thought to be a hepatocellular carcinoma cell line but shown to be from an hepatoblastoma (PubMed=19751877).

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: ENCODE project common cell types; tier 1.

Part of: JFCR45 cancer cell line panel.

Part of: MD Anderson Cell Lines Project.

Part of: TCGA-110-CL cell line panel.

Doubling time: ~50-60 hours (DSMZ).

Omics: Deep antibody staining analysis.

Omics: Deep exome analysis.

Omics: Deep phosphoproteome analysis.

Omics: Deep proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: Genome sequenced.

Omics: H3K27ac ChIP-seq epigenome analysis.

Omics: H3K27me3 ChIP-seq epigenome analysis.

Omics: H3K36me3 ChIP-seq epigenome analysis.

Omics: H3K4me1 ChIP-seq epigenome analysis.

Omics: H3K4me2 ChIP-seq epigenome analysis.

Omics: H3K4me3 ChIP-seq epigenome analysis.

Omics: H3K79me2 ChIP-seq epigenome analysis.

Omics: H3K9ac ChIP-seq epigenome analysis.

Omics: H3K9me3 ChIP-seq epigenome analysis.

Omics: H4K20me1 ChIP-seq epigenome analysis.

Omics: Metabolome analysis.

Omics: Protein expression by reverse-phase protein arrays.

Omics: Secretome proteome analysis.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

Omics: Virome analysis using proteomics.

STR信息

Amelogenin：X，Y；CSF1PO：10，11；D13S317：9，13；D16S539：12，13；D18S51：13，14；D19S433：15.2；D21S11：29，31；D2S1338：19，20；D3S1358：15，16；D5S818：11，12；D7S820：10；D8S1179：15，16；FGA：22，25；TH01：9；TPOX：8，9；vWA：17；

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