細胞名稱

人膀胱移形癌細胞RT112

貨物編碼

BFN60808321

產品規格

T25培養瓶x1

1.5ml凍存管x2

細胞數量

1x10^6

1x10^6

保存溫度

37℃

-198℃

運輸方式

常溫保溫運輸

干冰運輸

安全等級

1

用途限制

僅供科研        3類

培養體系

90%DMEM+10%FBS+1%雙抗 

培養溫度

37℃

二氧化碳濃度

5%

簡介

人膀胱移形癌細胞RT112取自女性供體，該細胞源于DSMZ

注釋

Part of: Cancer Cell Line Encyclopedia (CCLE) project.

Part of: COSMIC cell lines project.

Part of: TCGA-110-CL cell line panel.

Part of: UBC-40 urothelial bladder cancer cell line index.

Doubling time: 24 hours (PubMed=3708594); 25 hours (PubMed=25984343); 25.7 hours (PubMed=26055179); ~35 hours (DSMZ).

Microsatellite instability: Stable (MSS) (Sanger).

Omics: CNV analysis.

Omics: Deep exome analysis.

Omics: Deep quantitative proteome analysis.

Omics: Deep RNAseq analysis.

Omics: DNA methylation analysis.

Omics: shRNA library screening.

Omics: SNP array analysis.

Omics: Transcriptome analysis.

基因突變

FGFR3-TACC3 gene fusion (PubMed=25485619; PubMed=28855393).

TERT c.228C>T (-124C>T); in promoter (PubMed=24035680; PubMed=31068700).

Heterozygous for TP53 p.Ser183Ter (c.548C>G) and p.Arg248Gln (c.743G>A) (CCLE; Cosmic-CLP).

HLA信息

/

STR信息

Amelogenin        X

CSF1PO        10,11 (CLS; Cosmic-CLP; DSMZ; PubMed=27270441)

10 (PubMed=25877200)

D3S1358        15

D5S818        10,13

D7S820        11,12

D8S1179        13,15

D13S317        13,14

D16S539        11,13

D18S51        15 (CLS)

15,17 (PubMed=25877200)

D21S11        27,30

FGA        23 (CLS)

23,25 (PubMed=25877200)

Penta D        10,11 (CLS)

10 (PubMed=25877200)

Penta E        12,16

TH01        7

TPOX        8,11

vWA        14,17

參考文獻

PubMed=31068700; DOI=10.1038/s41586-019-1186-3

Ghandi M., Huang F.W., Jane-Valbuena J., Kryukov G.V., Lo C.C., McDonald E.R. III, Barretina J., Gelfand E.T., Bielski C.M., Li H., Hu K., Andreev-Drakhlin A.Y., Kim J., Hess J.M., Haas B.J., Aguet F., Weir B.A., Rothberg M.V., Paolella B.R., Lawrence M.S., Akbani R., Lu Y., Tiv H.L., Gokhale P.C., de Weck A., Mansour A.A., Oh C., Shih J., Hadi K., Rosen Y., Bistline J., Venkatesan K., Reddy A., Sonkin D., Liu M., Lehar J., Korn J.M., Porter D.A., Jones M.D., Golji J., Caponigro G., Taylor J.E., Dunning C.M., Creech A.L., Warren A.C., McFarland J.M., Zamanighomi M., Kauffmann A., Stransky N., Imielinski M., Maruvka Y.E., Cherniack A.D., Tsherniak A., Vazquez F., Jaffe J.D., Lane A.A., Weinstock D.M., Johannessen C.M., Morrissey M.P., Stegmeier F., Schlegel R., Hahn W.C., Getz G., Mills G.B., Boehm J.S., Golub T.R., Garraway L.A., Sellers W.R.

Next-generation characterization of the Cancer Cell Line Encyclopedia.

Nature 569:503-508(2019)

PubMed=31395879; DOI=10.1038/s41467-019-11415-2

Yu K., Chen B., Aran D., Charalel J., Yau C., Wolf D.M., van 't Veer L.J., Butte A.J., Goldstein T., Sirota M.

Comprehensive transcriptomic analysis of cell lines as models of primary tumors across 22 tumor types.

Nat. Commun. 10:3574-3574(2019)

PubMed=31978347; DOI=10.1016/j.cell.2019.12.023

Nusinow D.P., Szpyt J., Ghandi M., Rose C.M., McDonald E.R. III, Kalocsay M., Jane-Valbuena J., Gelfand E., Schweppe D.K., Jedrychowski M., Golji J., Porter D.A., Rejtar T., Wang Y.K., Kryukov G.V., Stegmeier F., Erickson B.K., Garraway L.A., Sellers W.R., Gygi S.P.

Quantitative proteomics of the Cancer Cell Line Encyclopedia.

Cell 180:387-402(2020)